The Effect of Hypotonic and Hypertonic Solutions on Fibroblasts of the Embryonic Chick Heart in Vitro

In the l i tera ture on tissue cul ture are repor ted , more or less completely, experiments test ing the effect of hypoton ic and hyper ton ic solutions upon growth of cells and upon individual cell s tructures. I t was wi th a view, therefore, of s tudying more fully the effect of solutions of different concentrat ions tha t the present work was undertaken. T h e experiments were performed in April, May , June, and July , when the weather was a t summer hea t and there was consequen t ly l i t t le danger of chilling the delicate new growths, which are susceptible to any sudden change of tempera ture . This po in t is impor tan t , because several invest igators have s ta ted definitely t ha t growth is much be t t e r in the spring and summer than in the fall and winter.